Standard nucleotide phosphoramidites include a dimethoxytrityl (DMT) protecting group on the 5’-position of the sugar base and a 3’-N,N-diisopropyl cyanoethyl group, which is essential for incorporation into oligonucleotide synthesis using the phosphoramidite strategy.
In the case of RNA, one more protecting group is needed at the 2’-position and most commonly a tert-butyldimethylsilyl (TBDMS) group is utilized. Finally, the nucleobases themselves (adenine, cytosine and guanine) require protecting groups, with the most commonly employed protecting groups being benzyl (Bn), acetyl (Ac) and isobutyryl (Ibu), respectively. All these protecting groups can be modified to match our clients’ specific needs.
Base and other modifications available upon request
- Deaza Phosphoramidites
- Halo Phosphoramidites
- Oxo Phosphoramidites
- Carboxy Phosphoramidites
- Spacer Phosphoramidites
- Linker Phosphoramidites
- Azido Phosphoramidites
- Fluorescent/dye labeled Phosphoramidites
- Locked nucleic acid (LNA) Phosphoramidites
- Morpholino Phosphoramidites
- Specialty Phosphoramidites
- 5-Me-dC, 5-Me-rU, 4-thio-rU
- 13C, 15N Phosphoramidites
- Deoxyinosine (dI), riboinosine (rI)