Bioconjugation Services

Our bioconjugation services include the following:
  • Design and synthesis of potent cytotoxic compounds, linkers, and payload-linker constructs.
  • Preparation and purification of bioconjugates from milligram- to gram-scale.
  • Modification of carbohydrates, polysaccharides, and glycoconjugates.
  • Preparation of fluorescent probes.

Conjugation methods employed at NJ Bio include:
  • Cysteine conjugation
  • Lysine conjugation
  • Conjugation to engineered cysteines and non-natural amino acids
  • Enzymatic conjugation

Toxins available at NJ Bio for the preparation of linker-payloads and use in conjugations include:
  • PBD dimer analogs
  • Duocarmycin analogs
  • Auristatin analogs
  • Maytansine analogs
  • Exatecan and SN-38 derivatives
  • Additional payloads

Express Conjugation Services using Lysine and Cysteine Conjugations: This expeditious service will require the client to supply the monoclonal antibody to NJ Bio. Amounts needed:
  • 5 – 20 mg of mAb for preparation of 3 – 10 mg of ADC.
  • Unused amounts of antibody will be returned to client.
Deliverables:
  • The well-characterized ADCs, typically with a drug-to-antibody ratio (DAR) in the range of 3 – 5, will be supplied in the desired formulation buffer.
  • ADCs will be characterized for: –  DAR by HIC or rp-HPLC and MS –  Monomer content by SEC –  Concentration by UV –  Residual linker-payload by LC-MS –  Endotoxin by LAL assay